Journal: bioRxiv
Article Title: Amyloid β induces cardiac dysfunction and neuro-signaling impairment in the heart of an Alzheimer’s disease model
doi: 10.1101/2023.07.11.548558
Figure Lengend Snippet: Top panels: Representative digital images (scale bar 50μm) showing endothelial cells (stained with CD31, in orange) and Aβ (in green). Bottom panels: representative digital images (scale bar 50μm) showing smooth muscle cells by α-Smooth muscle alpha-actin staining (αSMA, in red) and Aβ (in green) in cardiac sections from WT and Tg2576 mice. n=3-4 mice/group. WT mice are represented in the left panels and Tg2576 mice in the right panels.
Article Snippet: Via indirect immunofluorescence procedure, sections were analyzed using rabbit polyclonal antibodies against CD31 (AF3628-R&D Systems, 1:30) and α-smooth muscle actin (α-SMA, ab32575-Abcam, 1:500) at 4°C overnight, followed by specific secondary antibodies conjugated with cyanine 3 or cyanin 5 respectively, to stain vascular network.
Techniques: Staining